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After centrifugation at 13,000 rpm and 4 C for 15 min, the supernatant was collected and directly injected into the LCMS system for metabolomic analysis using U-C-fructose as an internal standard
First, SFN stands as a exemplary multi-targeted agent whose pleiotropic mechanismsspanning epigenetic regulation, induction of specialized cell death programs, immunomodulation, and CSC targetingprovide a robust foundation for its efficacy against heterogeneous and treatment-resistant malignancies
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